After attempting in vain to save a man from being mauled to death by a leopard, Jungle Jim discovers a vial containing an unknown potion. He takes it to district commissioner Marsden, who identifies it as being from the hidden temple of Zimbalu and brings in Dr. Hilary Parker to head up an expedition with Jim as her guide.
An opportunistic photographer, Bruce Edwards, follows as Dr. Parker seeks what she believes could be a miraculous breakthrough in medicine. Jim is more concerned that witch doctors use what's in the vial as a deadly poison. His trusty ally Kolu comes along and saves Jim when he is pushed off a cliff by Edwards. The favor is returned when Jim rescues Kolu from an attacking lion.
Accidents befall the expedition along the way, as Edwards ingratiates himself with the "devil doctors", only to later incur their wrath. The potion isn't the polio vaccine Hilary hoped it would be, but she expresses a willingness to work again with Jim in future adventures in the jungle.
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