After performing at a local club, trans women Rachel Slurr and Emma Grashun plan to party with Nacho and Chuey. When the guys tell them that they have a third friend, the girls convince their reluctant friend Bubbles Cliquot to join them. The girls drive to a warehouse where they are joined by Nacho and Chuey, along with their partner Boner. Bubbles tells her friends that Boner had recently drugged and raped her during a hook-up when he discovered she was a pre-op woman. Vowing to "finish the job", Boner, Nacho and Chuey attack the girls. Emma and Rachel are severely wounded but Bubbles is able to call her "mother" Pinky La'Trimm and Tipper Sommore and escape. Pinky and Tipper find Bubbles outside the warehouse and head inside to rescue Rachel and Emma. Instead, they are overpowered by the men and Boner attacks Bubbles again outside.
Some time later, Bubbles awakens from a coma. She learns from Pinky and Rachel that Emma and Tipper are dead. After Bubbles is discharged from the hospital, her friend Fergus trains the three surviving women in martial arts.
Back at Bubbles' apartment, Boner, Nacho and Chuey break in and overpower her again, tying her to a chair. Boner offers Bubbles a choice of ways to die but he momentarily turns his back on her. When he turns back Nacho and Chuey are unconscious and Bubbles, freed, knocks him out. A flashback reveals that Bubbles, Pinky, and Rachel set a trap for the men.
Boner regains consciousness to learn that the women have placed switchblade knives in the rectal cavities of Nacho and Chuey and a gun in his own rectum, weapons that will be triggered if any of them make any sudden moves. In a series of convoluted action sequences, Nacho and Chuey each recovers his knife and battles one of the women. Pinky kills Chuey and Rachel battles Nacho until Boner recovers his gun and holds it on Bubbles. Bubbles wrestles him for the weapon and, as Rachel finishes off Nacho, Bubbles shoots Boner in the chest. Pinky and Rachel each impale Boner with their thrown knives and Bubbles wrenches a large knife out of Nacho's skull and splits Boner's head with it, finally killing him.
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